The Leeds United centre-back, widely regarded as one of Craig Bellamy’s most dependable defensive options, played the full 90 minutes in Saturday’s 0-0 draw with Newcastle.
But despite coming through that match, Rodon has now been ruled out of Thursday’s game in Astana.
No details of the withdrawl have yet been given by the FAW, although it's understood the player is following concussion protocols.
Rodon’s absence deepens a growing injury crisis for Bellamy, with Wrexham pair Nathan Broadhead and Danny Ward, and Coventry full-back Jay DaSilva also withdrawing from the original squad.
To plug the gaps, goalkeeper Tom King, defender Rhys Norrington-Davies and Cardiff City midfielder Joel Colwill have been drafted in.
For Colwill, 20, it represents a first senior call-up and comes just days after his older brother Rubin was ruled out of the camp with an injury sustained in Cardiff’s victory over Plymouth.
Rodon’s absence is particularly significant.
Since making his debut in 2019, the 26-year-old has been a regular in the Wales back line and played a key role at Euro 2020 and in qualification for the 2022 World Cup.
His composure and leadership at the heart of defence will be missed in a game where Bellamy’s side are likely to come under sustained pressure.
Wales manager Craig Bellamy. Pic: Alamy
DaSilva also completed 90 minutes at the weekend as Coventry drew with Oxford but has since reported an issue that prevents him travelling.
Broadhead missed Wrexham’s win at Millwall with a calf injury, while Ward was stretchered off during that game and was always expected to miss international duty.
Bellamy was already navigating a challenging international window without Leeds midfielder Ethan Ampadu and Burnley full-back Connor Roberts, both sidelined, while captain Aaron Ramsey was left out of the squad due to limited game time at club level.
Colwill’s late inclusion offers a silver lining.
The Cardiff youngster has impressed with eight club appearances this season, and Bellamy had admitted last week that he was close to breaking into the senior setup.
Wales travel to Astana sitting second in Group J after collecting three wins from four qualifiers, their only setback a 4-3 defeat to Belgium in June.
They trail leaders North Macedonia by a point, with Belgium lurking in third but holding two games in hand.
Following the trip to Kazakhstan, Bellamy’s squad return home to face Canada in a friendly at Swansea’s Liberty Stadium on Tuesday, 9 September.
